Ludger's LT-MONO-96 kit is designed for the quantitative monosaccharide analysis of biopharmaceuticals and glycosylated media components within research and development, and quality-control labs.
The LT-MONO-96 kit contains the reagents and protocol for the release and fluorescent labelling of neutral and N-acetylated monosaccharides from therapeutic glycoproteins produced in mammalian expression systems, the isolated oligosaccharides or the cell-culture media.
The labelled monosaccharides can be analysed on standard HPLC systems using a Ludgersep R2 column or C18 column.
Accuracy and reliability are aided by robust chemistry, pure reagents and calibration using Ludger's Bioquant NIST-traceable external and internal quantitative monosaccharide standards.
The kit has been validated following ICH Q2(R1) guidelines and beta-tested in the field.
Precision values were said to be excellent compared with HPAE-PAD, GC-MS and other commonly used monosaccharide quantitation methods, with CVs of five to ten per cent across all monosaccharide species.
