Perfinity has integrated its automation and column technologies into Shimadzu instrumentation to create the Perfinity Workstation, a multicolumn apparatus that improves LC-MS analysis.
The Perfinity Workstation automates protein separations and mass spec sample preparation and is powered by Shimadzu hardware components that work in conjunction with five Perfinity-optimised columns.
Each column performs a step of the mass spec sample preparation process - affinity selection, buffer exchange, digestion, desalting and reverse-phase separation.
The automated integration of these steps enables users to start with serum and have peptides ready for LC-MS analysis in as little as 10 minutes.
The automated process removes much of the equipment and labour associated with the mass spec analyses of proteins.
The Perfinity Workstation combines the selectivity of antibodies with the resolving power of chromatography.
Buffer exchange and desalting are performed online.
The reduction in sample processing time enables users to screen a variety of conditions rapidly.
The advantage of the Perfinity Workstation's sample preparation approach is that users are not required to immobilise antibodies.
Instead, scientists can add antibodies directly to the sample.
By removing the immobilisation process, users eliminate a potential source of error, making LC-MS analysis with the Perfinity Workstation more accurate and reliable.
This platform is customisable across application areas including assay development, protein purification, drug development and biomarker discovery.
For example, methods built into the workstation automate core steps of assay development, limiting the amount of protocol optimisation required.
