454 Life Sciences, a Roche Company, and Nugen Technologies have announced the development of a sample-preparation method and high-throughput sequencing solution for small and degraded RNA samples.
The collaboration leverages 454 Life Sciences' capabilities to construct sequencing-ready cDNA libraries from RNA samples and Nugen's experience and innovation with genomic sample-preparation solutions.
This collaborative effort provides researchers with an end-to-end solution for transcriptome profiling using their most precious sample types.
Rare and difficult-to-obtain samples present a challenge to researchers who must maximise the utility of low-abundance sample.
Typical cDNA protocols often require up to 20ug of input material, which limits use to samples with large quantities of RNA available.
Under the collaborative relationship, the companies have developed a solution using Nugen's proprietary isothermal linear amplification (SPIA) products and the GS FLX Titanium cDNA Rapid Library Preparation protocol for the GS Junior and GS FLX Systems.
