Anaspec has launched the Sensolyte Fluorimetric NADP/NADPH assay kit, which detects NADP and NADPH without interfering with NAD/NADH.
NADP is converted to NADPH in an enzyme-cycling reaction.
The kit contains a fluorogenic reagent, producing fluorescence when reacted with NADPH.
The resulting red fluorescence can be monitored at excitation/emission of 560/590nm, with the intensity of fluorescence produced being proportional to NADP/NADPH concentration.
This assay detects as low as 1.5nM of the analyte.
Pyridine nucleotides are involved in a number of critical catabolic and anabolic reactions in living organisms.
Nicotinamide adenine dinucleotide phosphate (NADP) is a natural coenzyme present in all animal and plant organisms.
Cellular NADPH is important for tolerance to ROS and maintenance of cellular redox homeostasis.
The reducing power of NADPH is a required cofactor for enzymes that are prooxidant such as nitric oxide, synthase and NADPH oxidase.