Promega has launched the Gotaq one-step RT-qPCR system designed for the quantitative analysis of RNA.
The reagent system gives a bright signal to enable the early quantification of low- and high-copy RNA targets using a one-step reverse transcription quantitative PCR (RT-qPCR) protocol in a single tube format.
The system incorporates BRYT green fluorescent dye, which exhibits enhanced fluorescence when bound to dsDNA, and optimised buffer formulations to improve the data accuracy and sensitivity of low expressing targets or targets present at low amounts.
The Gotaq one-step RT-qPCR system is compatible with all real-time PCR instruments in both fast and standard cycling conditions.
The optimised formulations of the system components have been engineered to enable simple reaction setup and to provide consistent performance in all standard or fast instrument platforms.
The components and protocol enable cDNA synthesis using the Goscript reverse transcription system and quantification using the Gotaq qPCR Master Mix.
The Gotaq one-step RT-qPCR system can be used with any real-time instrument capable of detecting SYBR Green I or FAM dye and contains a low level of CXR reference dye.
A separate tube of CXR reference dye is included for use with instruments that require a higher level of reference dye.
