Promega has launched the Gotaq Long PCR Master Mix, an optimised enzyme mixture for improved long-range PCR.
The kit enhances PCR yield, sensitivity and specificity, and enables efficient amplification of up to 40kb from lambda DNA or 30kb from human genomic DNA.
The Gotaq Long PCR Master Mix components have been engineered with hot-start formulation to enable simple reaction setup and optimised to provide consistently efficient, accurate and robust performance of long DNA amplicons.
The master mix is suitable for cloning genes, mutational analysis and DNA sequencing.
The Gotaq Long PCR Master Mix contains Promega's Gotaq Hot Start Polymerase in a specially formulated mixture with a second proprietary thermal stable proofreading polymerase.
The presence of a proofreading enzyme to repair DNA mismatches in the presence of a highly processive polymerase allows increased DNA elongation, resulting in longer DNA amplification, according to the company.
Gotaq Long PCR Master Mix consists of a master mix containing the polymerase and proofreading enzyme mixture, dNTPs and MgCl2.
Also provided are control primers, human genomic control DNA and nuclease-free water.
Reaction setup requires the user to add their DNA template, primers and nuclease-free water to the master mix.
