Bio-Rad has launched two surface plasmon resonance (SPR) kits for use with the ProteOn XPR36.
The new kits — the novel hydrophilic ProteOn LCP liposome capturing kit and the lipophilic ProteOn GLC lipid kit — stably and selectively capture lipids and lipid assemblies for easier screening and analysis of membrane protein targets during drug discovery.
The ProteOn LCP sensor employs memLayer technology- the “native-lipid like” environment of the chip surface where proteins are captured ensures high quality data.
“The memLayer approach offers an interesting alternative,” said Maria Pavlaki, a senior researcher at Democritus University of Thrace in Greece. “The proteins can be in a more native-like environment (lipoparticles or liposomes) that resembles the cell membrane and are not ‘squashed’ on the surface.”
Capable of capturing multiple layers of lipid assemblies, the ProteOn LCP liposome capturing kit SPR kit is the first to allow label-free detection for binding analysis of peptides and small molecules. And, it empowers researchers to gauge drug absorption by monitoring interactions at the lipid’s surface and tracking uptake of molecules into the assembly.
The ProteOn LCP capturing reagent kit, included in the ProteOn LCP liposome capturing kit, is used to activate the LCP sensor chip with a biotinylated DNA tag that then hybridises to DNA-tagged lipid assemblies containing membrane proteins of interest.
The ProteOn GLC lipid kit provides researchers with a basic, easy-to-use option to study interactions of membranes with proteins and peptides.
Biopharmaceutical researchers use the kit to capture lipoparticles containing membrane protein drug targets of interest. Antibodies against those drug targets are then screened to measure binding kinetics, an important step in biotherapeutic development.
Researchers can customise the lipophilicity of the GLC sensor surface before capturing lipid assemblies (e.g. proteoliposomes and lipoparticles) by controlling the amount of alkylamine that is bound to the sensor surface.
This provides greater experimental flexibility and, because the surface is stable and regenerable, researchers have greater confidence in the quality of data collected over the course of a screening protocol.
