AnaSpec is providing a service for the generation of phospho and non-phosphospecific antibodies.
The functions of phosphorylation, the most prevalent of post-translational modifications, include the alteration of the activity of a substrate, its subcellular localisation, binding properties with other proteins.
To better understand phosphorylation and its functional role, the generation and use of phosphospecific antibodies is crucial.
For raising antibodies against phosphopeptides there are 3 critical factors for success: (1) the stability of the phosphorylation, (2) the purity of the peptide and (3) post-development purification of the phosphospecific antibody.
With documented experience in meeting all three factors, AnaSpec is able to maintain ISO9001:2008 manufacturing processes and robust quality validation.
The group’s peptide chemists routinely synthesise sequences containing single and multiple phosphoamino acids using technologies that are fine-tuned to produce high-yield, ultra-pure phosphopeptides.
Antibodies generated are then substantiated to be phosphospecific.
For polyclonal antibodies, researchers have a choice between two immunisation protocols - the Speedy 28-day protocol (an example of results is shown in Figure 2) and the 70-day standard protocol.
