NanoSight describes the work of Prof Tuan Vo-Dinh’s group at Duke University and their use of Nanoparticle Tracking Analysis.
The group is using Nanoparticle Tracking Analysis (NTA) to characterise metal nanoparticle construct materials for use in biosensing, imaging and cancer therapy.
The main research goal of the group is to develop advanced techniques and methods to protect the environment and improve human health.
As a part of these research goals, Dr Hsiangkuo Yuan and other members of Professor Vo-Dinh’s group design and fabricate metal nanoparticle constructs such as gold nanostar platforms.
These are characterised with UV-VIS, TEM, Raman microscope, fluorometers and other techniques.
However, to design nanoconstructs for in vivo applications, the particle size needs to be in the range from 10 to 100 nm for lower clearance from the kidney and reticuloendothelial system (RES).
It is important that the construct is in the right size range and is physiologically stable (non-aggregated) for biomedical applications in, for example, optical imaging or nanodrug delivery where it is also critical that the nanoparticle dose administered can be determined.
To compare plasmonic properties, i.e. the enhanced electromagnetic properties of nanoparticles, they need to determine the effect of different sizes and to understand in detail the profile of the particle size distribution of similar concentrations which can be obtained using NanoSight’s NTA system.
Prior to NTA, the group mostly used TEM to look at particle shape and measure particle size.
The surface coating or the aggregation state cannot be easily investigated using just TEM.
According to Duke University, NanoSight provides a significant complementary role on providing hydrodynamic size distribution and zeta potential.
Moreover, because NanoSight gives the concentration information, it allows them to normalise their comparison by individual particle counting which was quite difficult to obtain previously.