The Zetasizer µV applies both dynamic and static light scattering for size and molecular weight measurement.
Malvern Instruments’ detector range for gel permeation/size exclusion chromatography (GPC/SEC) now includes the Zetasizer µV.
The Zetasizer µV is a size and molecular weight detector that can be added to any system to boost experimental productivity by maximising information flow from a single experiment.
According to the company, it has particular value for protein characterisation.
It is easily connected to an existing GPC/SEC system using standard chromatography tubing and results reporting and analysis are handled through Malvern’s OmniSEC software.
With GPC/SEC the productivity of analysis is directly impacted by the number of detectors applied to the eluting, fractionated samples.
The Zetasizer µV uses dynamic light scattering to secure precise molecular size measurement while at the same time offering static light scattering for absolute molecular weight measurement.
In protein characterisation these capabilities support the detailed study of aggregation and conjugation and the flexibility to use the detector in cuvette mode is an important added bonus- one that allows the characteriaation of discrete samples with just 2 µL of material.
Features overview:
- Users can switch in seconds from chromatography detector to cuvette mode
- Precise temperature control
- Sensitivity - 0.1mg/mL 15kDa protein (Lysozyme)
- Only 2µL sample required in cuvette mode to preserve sample
- 8µL flow cell for chromatography mode ensures minimal band broadening to preserve the resolution of the separation
- Automated temperature trends for aggregation temperature determination
To download the Zetasizer µV product brochure, please click on the link above.
