Ludger has developed labelling kits containing the non-toxic reducing agent 2-picoline borane (2-PB).
Fluorescent labelling methods for glycoprofiling of therapeutic glycoproteins are well established and aid subsequent separation and quantitation using a range of techniques.
These include high performance liquid chromatography (HPLC and UHPLC), capillary electrophoresis (CE), and mass spectrometry.
Of the fluorescent labels available, 2-aminobenzamide (2-AB) and 2-aminobenzoic acid (2-AA) are most widely used for oligosaccharide profiling.
However, existing 2-AA and 2-AB labelling kits use highly toxic sodium cyanoborohydride as a reducing agent during glycan labelling.
Following on from research performed by Ludger in collaboration with Leiden University Medical Centre, The Netherlands, Ludger has developed labelling kits containing a different and non-toxic reducing agent, 2-picoline borane (2-PB). T
The kits have been validated following ICH Q2(R1) guidelines and beta-tested in the field.
The kits offer equivalent labelling efficiency as standard LudgerTag 2AA and 2AB labelling kits containing sodium cyanoborohydride.
Each kit comprises two premixed bottles; one containing acetic acid and DMSO solution and the other containing 2-AB or 2-AA label combined with 2PB. This makes the labelling process simpler.
