The CFX qualification plate claims to accurately verify the performance of any CFX real-time PCR system.
Preloaded with an optimised master mix containing Bio-Rad’s SsoAdvanced SYBR Green supermix, the CFX qualification plate provides a five-point standard curve, two unknown groups, and no-template controls.
This allows for the rapid determination of a pass/fail instrument performance result.
Included in Bio-Rad’s CFX Manager software is the new qualification plate check tool for analysing the CFX qualification plate data.
The tool uses an algorithm to determine a pass/fail performance result based on two criteria: twofold discrimination with a 99.7% confidence level and single-product melt peaks for the two unknowns.
A passing result indicates good optical performance and uniform thermal block control.
Features overview:
- 3-step run start workflow with predispensed plate, predefined thermal cycling protocol, and plate templates
- 1-click pass/fail performance result with CFX Manager software
- 2-fold discrimination with 99.7% confidence level and single product melt peak criteria for determining pass/fail results
- Easy incorporation into an instrument qualification procedure with a printable report