The system enables bisulfite conversion and DNA clean-up within two hours while maintaining DNA integrity.
MethylEdge requires only small amounts of input genomic DNA (100pg-2µg).
Using optimised conversion reagents, clean-up and recovery methods, the system converts DNA at =90% efficiency with minimal fragmentation.
The resulting DNA is suitable for use in downstream applications such as PCR, array, or sequencing assays.
The kit includes all reagents necessary to perform 50 bisulfite conversion reactions.
All MethylEdge reagents can be stored at room temperature and require minimal up-front preparation.
Control DNAs are also available separately and can be used as standards in both the conversion reaction and post-conversion analysis.
The Promega MethylEdge Bisulfite Conversion System complements Promega nucleic acid purification kits for a wide variety of sample types and throughput levels, quantitation chemistries for both pre- and post-conversion, and Promega amplification technologies for detection.
