Porvair Sciences has announced the addition of the novel Chromatrap chromatin immunoprecipitation (ChIP).
Developed in conjunction with the Department of Life Sciences at University of Swansea, Chromatrap uses a solid phase porous polymer functionalised with protein A, allowing the chromatin capture to be more efficient than bead based methods.
The Chromatrap spin column approach offers significant advantages compared to methods based on sepharose or magnetic beads, which involve many steps of separation, pipetting and re-suspension.
Independent tests demonstrate levels of DNA pull down up to 25 times higher than with traditional bead based procedures, excellent DNA enrichment with signal-to-noise ratios typically 2 to 3 times better than competing procedures and top performance even with samples as small as 50ng or as much as 3000ng chromatin per immunoprecipitation.
Features overview:
- Column loadings extended to include 50ng-3000ng chromatin per I.
- Reduced incubation times.
- ChIP assay can be reduced from 24hrs to 5hr.
- Improved elution chemistry no longer includes urea.
To learn more about the system, please click on the link above.
