Bioo Scientific has introduced the NEXTflex qRNA-Seq Kit for high precision gene expression analysis.
Developed in conjunction with Cellular Research, the kit is designed to generate libraries equivalent to conventional RNA-Seq libraries, but with the added feature of Molecular Indexing technology.
Similar to conventional RNA-Seq, sample RNA is converted to cDNA fragments. But prior to any PCR amplification steps, all DNA fragment ends are ligated to a pair of adaptors chosen at random from a total set of 9,216 molecular indices.
Individual DNA molecules of identical sequence become distinct through indexing, allowing for differentiation between re-sampling of the same molecule and sampling of a different molecule of identical sequence.
Analysis using molecular indexing information provides an absolute, digital measurement of gene expression levels, irrespective of common amplification distortions observed in many RNA-Seq experiments.
All of the NEXTflex kits incorporate the proprietary ’Enhanced Adapter Ligation Technology’, which claims to offer the highest ligation efficiency available, resulting in longer and more diverse sequencing reads.
