PerFix-Expose and PerFix-nc reagent kits for intracellular staining minimise sample manipulation for a streamlined workflow that delivers high quality results.
Developed for use on Beckman Coulter’s Gallios and Navios series flow cytometers and compatible with the company’s fluorochromes, the kits enable a clear focus on the cytoplasmic and nuclear compartments of the cell.
PerFix-Expose helps scientists identify and explore complex intracellular signalling pathways disrupted by cancer and other diseases.
It allows fluorochrome-coupled antibodies and other probes to enter into cells, their nuclei and other cell compartments, where they bind to proteins relevant to signalling pathways that have been disrupted by disease.
With PerFix-nc (no centrifugation), researchers can perform concomitant surface and intracellular staining for flow cytometry to take advantage of a new approach that provides easy access to the inside of human leucocytes.
Analysis of multiple intracellular epitopes, or multiple intracellular markers along with cell surface markers, is accomplished by straightforward fixation, permeabilisation and staining of leucocytes from peripheral whole blood, together with erythrocyte lysis.
PerFix-nc kit quality is characterised by mean fluorescence intensities and consistent performance in repeatability studies, as demonstrated by levels of cell recovery.
The kit offers an original and powerful leucocyte permeabilisation method followed by an optimised fixation step in a process that involves only 15 minutes of hands-on user time.