Acrylamide kits from Bio-Rad give handcast gel users access to the latest PAGE gel technology and TGX precast gel benefits.
Bio-Rad Laboratories has launched TGX FastCast acrylamide and TGX Stain-Free FastCast acrylamide kits, giving handcast gel users access to TGX gel chemistry, previously available only in precast gels.
The TGX FastCast kits allow users to hand cast polyacrylamide gels with fast run times, efficient protein transfers, and consistent, reproducible results.
Researchers can also take advantage of the TGX Stain-Free version, which allows users to monitor the success of each step in their protein electrophoresis and western blotting workflows.
Unlike traditional handcast gels, which require 60 minutes to polymerise before running, FastCast gels can be used only 30 minutes after casting.
Protein electrophoresis can then be run on these gels in as little as 20 minutes.
As with Bio-Rad’s TGX precast gels, proteins can be transferred from FastCast gels in as little as 3 minutes when used with the company’s Trans-Blot Turbo transfer system. FastCast gels can be stored for up to four weeks after casting, allowing multiple gels to be cast at once for use as needed.
The TGX Stain-Free FastCast acrylamide kits allow protein detection in gels or on membranes in 3–5 minutes using Bio-Rad’s stain-free imaging systems.
This makes it possible for researchers to cast their gels and achieve complete protein separation, gel imaging, and data analysis in less than an hour.