An instructional video from Porvair Sciences explains how to conduct a ChIP assay for epigenetic research starting from a confluent monolayer of human cells.
The live demonstration of the Chromatin Immunoprecipitation (ChIP) assay uses Porvair’s solid-state ChIP kit – Chromatrap 96.
The video was prepared to inform researchers of an optimised protocol for preparing fresh chromatin from a dish of growing cells and how to verify the quality of the chromatin recovered.
In addition each simple step in the Chromatrap procedure is shown, leading to measurable amounts of high-purity chromatin ready for PCR or sequencing.
Because Chromatrap does not use beads to separate the chromatin, Porvair claims that it is faster, easier to use and less prone to manual handling errors.
The video is designed to show this, as well as demonstrating simple centrifugation and wash steps on the immunocaptured chromatin, which is held on the novel solid-state disc at the heart of each Chromatrap spin column.
This video is aimed at those contemplating the technique, but who have yet to perform a ChIP assay.
In addition the video it provides users of traditional bead-based methods with the opportunity to see for themselves how much easier and quicker their ChIP assays can become in the lab by using Chromatrap spin columns or 96-well filter plates.