Thermo Scientific high-res accurate mass LC/MS system designed to scan 50% faster than predecessor.
Thermo Scientific has unveiled the latest addition to its Q Exactive family of Thermo Scientific liquid chromatography-mass spectrometry (LC/MS) systems, the Thermo Scientific Q Exactive HF instrument.
To create the Q Exactive HF system, Thermo Fisher Scientific added an ultra-high-field Orbitrap mass analyser to its Q Exactive Plus LC-MS platform.
It is designed for faster identification, characterisation, quantification, and confirmation in a single, high-confidence analysis, the company says.
Maximum scanning rate increases from 12 Hz to 18 Hz which significantly accelerates productivity, Thermo claims.
Company-conducted experiments have demonstrated the Q Exactive HF can provide complete MS/MS identification for 18 scans in one second, compared to half that number using the previous Q Exactive systems for the same duration gradient, without compromising data quality.
Maximum resolution is 240,000 at m/z 200.
“For shotgun analysis of human proteomes, we now can obtain the same data in half the time. We can therefore analyse many more cell conditions than before,” said Jesper Olsen of the Novo Nordisk Foundation Center for Protein Research, an early-access user.
Advanced Quadruple Technology is designed to optimise precursor selection and transmission for excellent detection of low-abundance ions in very complex matrices.
Quantitation capabilities include: selected ion monitoring, parallel reaction monitoring and data-independent acquisition.
An optional intact protein mode enhances analysis of intact proteins.