Porvair Sciences has published a guide to help laboratories choose how best to verify the quality and quantity of their Chromatin Immunoprecipitation (ChIP) DNA.
The guide reports on experiments comparing the performance of the Thermo Scientific NanoDrop 2000, the Life Technologies Qubit 2.0 fluorimeter and the Agilent Bioanalyzer 2100 for qualitative and quantitative analysis of double stranded immunoprecipitated DNA.
The advantages and limitations of detection sensitivity, reproducibility and automated analysis of each instrument are covered, along with the compatibility of each method with downstream applications including next generation sequencing.
With continuing evolution in epigenetics, molecular biology and biochemical research, the time and cost of performing basic laboratory techniques has grown in importance.
One of the most common and often laborious measurements in bioscience labs is that of DNA quantification and characterisation.
DNA may be quantified for applications including next generation sequencing (NGS), PCR amplification and plasmid transfection.
Accuracy of DNA quantification can depend on the technology used and whether it is the most appropriate for the sample type being quantified.
For a copy of the guide please visit www.chromatrap.com or contact Porvair Sciences on 44-1978-666239 or int.sales@porvairsciences.com.
