The PrimeQ real-time Polymerase Chain Reactor (PCR) System is a feature-packed instrument that has been designed with an open architecture and chemistry format in order to give researchers flexibility in the use of qPCR methods and, hence, in their research goals.
At the heart of the system is a photomultiplier tube detector (PMT) – a highly accurate method of measuring photons with some advantages over CCD systems where light is measured from a digital image, Techne says.
The PrimeQ therefore does not rely on internal algorithms to compare different images, but can analyse the raw data produced from the PMT.
The PrimeQ is equipped with a single, long-lasting, white LED power source. This ensures a consistent light to each well when compared to multiple LED systems or comparatively short-lived tungsten halogen lamps.
The PrimeQ has multiplex capabilities with four paired excitation and emission filters housed with an individual cartridge system.
The open system also means that the PrimeQ is not locked in to factory calibrated dyes. The accurate flexible scanning mechanism will allow for both full (20 seconds per filter) and partial plate reads with no cross talk between wells.
The temperature controlled, heated lid of the PrimeQ will minimise sample loss and prevent contamination.
It also allows pre-incubation stages to be further optimised in methods such as reverse transcription or where different stages of a programmed run have different temperature regimes.
And all of these features on the PrimeQ come with Quansoft software and reporting - an easy-to-use software package that gives the operator complete freedom to manipulate the raw data generated.
PrimeQ analysis includes ’Baseline’ a method permitting correction of differences in background fluorescence.
Dissociation curve measures the melting temperature, the temperature at which dsDNA denatures.
Quantification determines the absolute or relative quantity of a DNA template by measuring cycle-to-cycle fluorescence changes, while plus/minus scoring determines with ease and accuracy the presence/absence of a PCR product in a sample.
Allelic discrimination is a powerful technique that detects a single nucleotide polymorphism while ’multi-read’ is an end point analysis method that reports the average fluorescence of a selected number of readings.
PrimeQ is real-time PCR, with real differences, and real advantages.