The offline combination of liquid chromatographyseparation with Maldi-MS provides significant advantages over the more widespread approach of online electrospray ionisation
Agilent Technologies has announced its new 1100 series liquid chromatography (LC) micro collection/spotting system, a complete software-controlled separation solution for proteomics that features direct spotting on matrix-assisted laser desorption/ionisation mass spectrometry (Maldi-MS) targets.
The system provides flexibility for multidimensional separations and allows users to combine the benefits of chromatography with the power of Maldi-MS.
The micro collection/spotting system fixes the chromatographic separation on the Maldi plate.
The offline combination of LC separation with Maldi-MS provides significant advantages over the more widespread approach of online electrospray ionisation.
MS/MS experiments can be performed without time constraints and decisions can be made during the experiment, allowing results-driven analysis without rerunning the sample.
Separation performance and total sample throughput are optimised by decoupling the separation from the analysis.
The micro collection/spotting system is designed as a separation tool for proteomics.
Based on the industry-leading Agilent 1100 series high-performance liquid chromatography system, the system enables separation of small sample amounts at low flow rates for the subsequent collection of very small fraction volumes.
The offline approach produces superior separation results for enhanced protein identification and allows researchers to perform enzymatic or chemical modifications of fractions between separation runs.
The Agilent 1100 series LC micro collection/spotting system is available now.
