The procedure detects and identifies oligoclonal IgG bands in the isoelectrophoretic patterns of unconcentrated human cerebrospinal fluid samples
Available through exclusive UK distributor Analytical Technologies, Sebia has introduced CSF isoelectric focusing for its Hydrasys semi-automated electrophoresis systems in clinical immunology, biochemistry, and protein reference laboratories.
The CSF testing capability can be supplied as part of a new instrument or as an upgrade to existing Hydrasys systems.
The procedure detects and identifies oligoclonal IgG bands in the isoelectrophoretic patterns of unconcentrated human cerebrospinal fluid samples.
Equivalent amounts of paired serum and CSF protein samples are run simultaneously, and are firstly separated by isoelectric focusing across a pH gradient at 1000V to achieve better resolution.
Oligoclonal IgG patterns are then detected using immunofixation with an enzyme-labelled IgG antiserum and colour substrate to allow the separation of IgG in serum and CSF to be compared.
The CSF kits contain pre-prepared purified agarose gels with ampholyte, six or 18 sample applicators, two pre-prepared pH buffers, and developing reagents for final detection.
The easy to use kits ensure reliable results and eliminate the time-consuming nitrocellulose immunoblotting and milk washing procedures.
Although oligoclonal banding is neither diagnostic nor specific for multiple sclerosis, it is considered an essential test for the detection of Intrathecal IgG synthesis and inflammatory profiles by the 1994 consensus of the Committee of the European Concerted Action for Multiple Sclerosis.
