Automated counter relies on a fluorescence microscopy technique for counting cell nuclei stained with propidium iodide, combined with a CCD camera
Cell culture scientists usually employ microscopic techniques to determine cell concentrations.
Done manually, this approach is often time-consuming and can lead to results that are dependent on the subjective judgment of the researcher.
In addition, samples from cell cultures often show a tendency to form cell aggregates, which makes it difficult to count individual cells. To address these problems, ChemoMetec has developed the NucleoCounter, which relies on a fluorescence microscopy technique for counting cell nuclei stained with propidium iodide. Combined with a CCD camera and integrated image analysis, the compact microscope was designed to allow the small and large labs to perform fast and automatic cell counts.
To use the NucleoCounter, a suitable volume of the sample is first mixed with a volume of lysis/disaggregation buffer followed by stabilising buffer and mixed for a few seconds.
The NucleoCassettes is operated by immersing the tip of the cassette into the sample mixture and pressing the load button; this loads predefined volume of the sample mixture into the cassette and is stained with PI.
Results are available in 30 seconds and displayed on the NucleoCounter.
Alternatively total counts and viability counts are presented on the NucleoView software together with signal histogram and complete image picture.
All results can be transferred to a spreadsheet for further manipulations.
