These nanometre sized semiconductor crystals provide a superior detection platform for nucleic acid and protein analysis
New additions to the Quantum Dot range are now available from Cambridge BioScience for fluorescent biolabelling - QdotT 605 and 655 biotin conjugates.
These nanometre sized semiconductor crystals provide a superior detection platform for nucleic acid and protein analysis.
Biotin covalently attached to a bright Qdot nanocrystal can be used to detect streptavidin, neutravidin and avidin in a variety of biological applications such as plate assays, immunofluorescence microscopy and flow cytometry.
The avidin-biotin interaction is one of the highest affinity interactions known in any biological system.
Qdot biotin conjugates exhibit very high specific and reduced non-specific binding.
Excitation is flexible with varied light sources and their narrow emission permits simple multiplexed detection with colour filters.
Qdot biotin conjugates can be used together with Qdot streptavidin conjugates for further signal amplification.
As with all Qdots, the intense signal is photostable providing plenty of time for imaging and analysis.
No cyto-toxic anti-fade reagents are required and reanalysis is equally as quantitative as initial analysis.
Even low abundance targets can be accurately quantitated.
A variety of fluorescence instruments can be used for the detection of Qdots with no hardware modification.
These products are available from Cambridge BioScience in the UK and Ireland only.
