Light emitted from the chemical reaction is directly proportional to the amount of expressed enzyme and thus the binding activity of the targeted transcription factor
TransLucent reporter vectors for monitoring and quantifying the binding activity of transcription factors (TF) are now available from Cambridge BioScience.
Developed by Panomics, the vectors can also be used to validate the results obtained from TranSignal protein/DNA arrays.
Results are rapid and for increased flexibility these assays can be performed in vivo.
Each TransLucent reporter vector contains a cis-acting DNA binding element that is recognised by a specific TF.
Binding at this site results in the expression of luciferase, an enzyme capable of catalysing a powerful bioluminescent reaction.
Light emitted from the chemical reaction is directly proportional to the amount of expressed enzyme and thus the binding activity of the targeted TF.
As a means of measuring promoter response in cells, the luciferase assay is simple, straightforward, and very effective. The luminescence from this chemical reaction can be read and quantified by a luminometer or scintillation counter and the amount of light detected correlates directly with the binding activity of the transcription factor.
These products are available from Cambridge BioScience in the UK and Ireland only.
