Among other applications, they can be used to evaluate uncharacterised growth factors, extracellular stimuli, and upstream events in the NFkB and Stat3 signalling pathways
Cambridge BioScience now offers Panomics NFkB and Stat3 stable reporter cell lines for high throughput analysis of in vivo drug efficacy and specificity.
These cells also provide a reproducible, ready-to-use method of performing cell-based assays.
Among other applications, they can be used to evaluate uncharacterised growth factors, extracellular stimuli, and upstream events in the NFkB and Stat3 signalling pathways.
NFkB stable reporter cell lines are available in human 293T and A549 cells and the Stat3 stable reporter cell line in HeLa cells. Nuclear Factor kappa B (NFkB) is a member of the rel family of transcription factors and plays a key role in the regulation of inflammatory response, apoptosis or tumorigenesis.
With Panomics's NFkB reporter stable cell line you can accurately monitor any changes occurring along the NFkB pathway, says Cambridge Bioscience.
The stable cell line is derived from human 293T cells with chromosomal integration of a luciferase reporter construct regulated by six copies of the NFkB response element. Signal transducers and activators of transcription (Stats) are transcription factors that are phosphorylated by JAK kinases in response to cytokine activation of cell surface receptors.
Stat3 induces progression through the cell cycle, prevents apoptosis, and upregulates oncogenes, such as c-myc and bcl-X.
Stat3 has also been shown to play a critical role in hematopoiesis.
The Stat3 stable reporter cell line maintains chromosomal integration of a luciferase reporter construct (pStat3-Luc) regulated by three copies of the Stat3 response element.
These products are avialble from Cambridge BioScience in the UK and Ireland only.
