Purified DNA from clotted blood is said to be easy to achieve using this quick protocol, involving little more than centrifuging
Clotspin enables the user to obtain a yield of 5-20µg DNA/ml clot
using a very quick and easy protocol.
Clotspin is unique and
unlike any other product on the market.
Just add a clotted blood
sample to the Clotspin disposable basket and centrifuge.
discard the basket and proceed with a modified Puregene protocol
Clotspin, manufactured by Gentra Systems and
distributed by Flowgen saves time and money.
There is no need to
cut and form mesh.
Clotspin is very efficient and increases
productivity, which directly translates to greater savings
compared to other mesh protocols or other methods using excess
Also, shorter Proteinase K digests are necessary
since the clot is broken up into smaller pieces, saving even more
The Puregene kit enables recoveries of high quality DNA to
at least 100kb in size (absorbency ratio of 1.7 to 2.0),
consistently high yields of up to 140µg of DNA from a 5-7ml clot
Puregene effectively removes PCR inhibitors and
other contaminants making the DNA suitable for a wide variety of
down stream applications, including Southern blotting and PCR.
All reagents (except alcohol) are provided at the correct
dilution and the manipulation steps are minimised and there is no
requirement for columns or glass beads and no phenol or
chloroform is necessary.
A variety of isolation protocols and
technical reports offering hints and tips plus additional
information are available free from Flowgen.