Assay reagent enables quick and reproducible absolute cell count and viability assessments with insect and other non-mammalian cells
Guava Technologies has released a technical paper detailing how its ViaCount Flex assay reagent enables quick and reproducible absolute cell count and viability assessments with insect and other non-mammalian cells.
Guava ViaCount Flex provides a measure of speed, ease of use and reliability to cell counting that was not previously available to researchers using non-mammalian cell lines.
Developed to address the special needs of researchers working with non-mammalian cell lines, in particular insect cells, ViaCount Flex can help accelerate work in vaccine research and commercial vaccine production, as well as in studies of insect pathology and disease states, all of which increasingly rely on insect cell cultures.
Created specifically for use with insect and non-mammalian cell cultures, which often have biological characteristics that make them harder to analyse, ViaCount Flex produces assay results that are highly reproducible, and there is significantly less operator-to-operator variability in results compared to manual counting methods.
Moreover, the Guava ViaCount Flex assay is easy to perform, with rapid data acquisition on the Guava PCA system, saving researchers significant amounts of time.
In addition to the speed and simplicity of Guava ViaCount Flex, results from ViaCount Flex assays show good correspondence and linear correlation to the current standard cell counting method, trypan blue hemacytometer counting, over a broad range of cell densities and culture conditions.
Copies of the technical paper describing validation studies comparing Guava ViaCount Flex assays of Sf9 insect cells with traditional counting methods using trypan blue staining and a hemacytometer are downloadable from the Guava Technologies online library.
