Luciferase reaction has an extended half-life of over five hours giving great flexibility when carrying out luminescence measurements
Promega's CellTiter-Glo luminescent cell viability assay is a one-step homogeneous protocol combining unsurpassed sensitivity, ease and accuracy.
The assay determines the number of viable cells in culture by quantifying the amount of ATP present - a marker of metabolically active cells.
The assay is designed for 96 or 384 well formats, making it ideal for automated high throughput screening (HTS), of cell proliferation and cytotoxicity assays.
A homogeneous 'add, mix and measure' format makes the CellTiter-Glo assay simple to use with no cell washing, media removal or multiple pipetting steps. Measurements can be made on a CCD camera imaging device or a plate reading luminometer (no injector is required) as little as 10 minutes after addition of the reagent.
Long incubations are not necessary.
The CellTiter-Glo assay exhibits excellent sensitivity, accurately measuring cells at numbers below the detection limits of standard colourimetric and fluorometric assays.
As few as 15 cells can be detected meaning that less cells are required per assay, a real plus when cells are at a premium.
This luciferase reaction has an extended half-life of over five hours giving scientists great flexibility in when they choose to carry out luminescence measurements.
Using the CellTiter-Glo assay, laboratories can precisely measure cell viability in continuous or batch processing.
The CellTiter-Glo assay has an improved safety profile over classic viability assays, such as [3H] Thymidine incorporation assays, as handling of radioactive isotopes is avoided.
