New system claimed to be the first homogeneous assay to enable the sequential measurement of stable bioluminescence from two reporter genes in a single sample
The Dual-Glo Luciferase assay system from Promega is described as a breakthrough for HTS applications.
This new system is the first homogeneous assay to enable the sequential measurement of stable bioluminescence from two reporter genes in a single sample.
Using the Dual-Glo assay, researchers are able to accurately control for expression variability - so reducing false positives and improving confidence in the hit rate of a screening programme. Controls can be established for cell number, cell health, transfection efficiency and non-specific cellular responses.
This robust and sensitive dual reporter assay is more predictive of therapeutic efficacy, increases the reliability of the data and provides high Z-factors.
This simple 'add-and-read' system generates both firefly and Renilla luciferase bioluminescence signals from cells assayed directly in growth medium.
The simple, two-step assay is ideal for automation with robotics.
Each luminescent signal may be measured for up to two hours after reagent addition, enabling batch and continuous processing of 96, 384, and 1536 well plates for greater throughput.
Both reporter reactions are linear over at least six logs of enzyme concentration, permitting analysis of high and low reporter activity without sample dilution.
The Dual-Glo Luciferase assay system offers researchers in pharmaceutical discovery, biotechnology and basic sciences an easy-to-use and accurate tool that yields high quality data and is amenable to the analysis of tens of thousands of samples using automation.
