Designed to give control of variables in microarray fabrication and experimental processing with significantly reduced workload as reagent preparation is already completed
A range of integrated systems designed to give researchers the ability to control variables in microarray fabrication and experimental processing has just been launched.
These six Pronto Plus systems are the first products to emerge from the business alliance between Corning and Promega, which was announced in October 2003.
By combining key technologies from both companies, Pronto Plus systems significantly reduce workload for the researcher as all reagent preparation, validation and optimisation is already completed.
Furthermore, the integrated system approach presents a cost saving over buying separate reagents.
Through standardised production methods and stringent QC control, interslide variation of less than 10% is achieved. This provides greater confidence for researchers to be able to compare data from different experiments, and even different laboratories.
Protocols in all of the Pronto Plus systems are simple and easy to follow meaning that researchers can manipulate RNA and microarrays quickly and easily, regardless of their level of experience.
Stringent quality control in the production of the systems ensures consistency of reagents, enabling a sensitivity level of one copy per cell in 5x10^5 cells.
Reagents have been optimised to conserve samples; just 5ug of total RNA per labelling reaction, or 1.5ug of Poly(A)+RNA is required.
There are currently six configurations of system, each tailored to match specific researcher needs.
Two systems are intended for core facilities to validate Pronto reagents from microarray manufacture through to hybridisation.
A further two systems are designed to purify total RNA, generate labelled cDNA and hybridise to microarrays printed on UltraGaps slides.
The final two systems are configured for researchers who already have purified total RNA but need to label cDNA prior to hybridisation to microarrays printed on UltraGaps slides.
