Updated instrument has software enhanced by the inclusion of target factor analysis to determine the pKa results by automatically deconvoluting multiwavelength UV spectra
Sirius Analytical Instruments has recently introduced its updated ProfilerSGA instrument for high throughput measurement of pKa values.
Knowledge of pKa is the key to understanding how other properties of a drug molecule change with respect to pH.
With ProfilerSGA, scientists can enhance their physicochemical profiling for the assessment of ADME properties of newly discovered drugs.
In the updated instrument, the software has been enhanced by the inclusion of target factor analysis (TFA) to determine the pKa results by automatically deconvoluting multiwavelength UV spectra.
A new brochure details how the instrument employs a novel patented UV gradient titration technique and proprietary buffer solutions (which remove the need for a pH electrode) to measure pKa rapidly.
Additional information about ProfilerSGA can be downloaded from the Sirius web site free of charge, including a presentation in pdf format called 'Improving throughput from physicochemical assays to better assess bioavailability earlier in drug R and D'.
This presentation examines how the physicochemical properties log P, solubility and permeability are all dominated by the pKa values for ionisable compounds.
The presentation also discusses high throughput instrumentation that is available to measure all these parameters.
