Revolutionary high-performance benchtop quadrupole time-of-flight mass spectrometer is said to outperforms typical floor-standing Q-q-TOF systems
Bruker Daltonics has announced what it calls a revolutionary new ESI-Q-q-TOF mass spectrometer, the Microtof-Q.
This compact benchtop instrument claims market-leading resolution of 15,000 at full sensitivity, ie without any W- reflection and the associated ion losses.
It also features routine, robust 3ppm mass accuracy in MS and MS/MS scans over a wide dynamic range.
Due to its new Apollo II ion funnel ESI source, the Microtof-Q is said to offer outstanding sensitivity.
Moreover, its high dynamic range and use of modern electronics and detector technology make it highly suitable for quantitation in proteomics and small molecule analysis.
The Microtof-Q offers SigmaFit precise isotopic profile analysis technology for what is said to be unprecedented confidence in molecular and fragment identification.
SigmaFit analysis is available under automation on-the-fly on both MS and MS/MS spectra as a third dimension for molecular formula generation and identification, which is orthogonal and complementary to accurate mass determination, and to MS/MS fragment information.
Together, better than 3ppm accurate mass and SigmaFit provide near-certainty in identification, whereas accurate mass by itself still can leave considerable ambiguity as to the exact sum formula of a molecule or fragment, says Bruker.
The combined analysis offers new levels of specificity in life- science mass spectrometry.
The Microtof-Q by design offers fundamentally robust mass accuracy over a high dynamic range to ensure confident mass assignments on both trace and abundant signals simultaneously.
Its outstanding speed and 20 full spectra/sec acquisition rate make it ideal for very fast liquid chromatography in proteomics, biomarker discovery and metabolomics.
Market-leading resolution is provided across the entire mass range at maximum sensitivity, allowing the scientist to routinely utilise the instrument's full potential on all samples.
Michael Schubert, vice president of R and D at Bruker Daltonics, explained: "Innovative design drives the Microtof-Q to new standards of performance and convenience.
"Our new Apollo II ion funnel source raises sensitivity up to ten-fold, and its speed and dynamic range are unprecedented in previous generation Q-q-TOF systems.
"As a next-generation ESI-Q-q- TOF instrument, the Microtof-Q offers leading performance in all aspects and sets a new standard in resolution at full sensitivity, yet it is the most compact design anywhere with a footprint of only 64cmx94cm and the convenience of a bench-top design".
"We believe the Microtof-Q provides the life-science researcher with a unique new discovery and validation tool that represents dramatic progress in life-science mass spectrometry," added Frank Laukien, president and CEO of Bruker Daltonics.
"Our unique SigmaFit technology combined with outstanding resolution and accurate mass performance, allow the Microtof-Q user to identify or confirm molecules and their fragments with unprecedented specificity and confidence.
"In summary, we believe that the Microtof-Q represents next-generation Q-q-TOF technology that will change the market paradigm, and will drive the ESI-Q-q-TOF market mostly towards convenient, yet extremely powerful benchtop systems".
The Microtof-Q seamlessly integrates into various available system solutions: ProteinScape, the proteomics bioinformatics suite which was selected by the Hupo Brain Proteome project as their official worldwide tool, the Proteineer solution for LC and 2D gel-MS/MS based expression proteomics projects, and the Metabolic Profiler solution for metabolomics (with optional full integration into a Bruker Biospin LC/NMR-MS platform).
For pharmaceutical customers, the Microtof-Q offers software tools to support customers in achieving 21 CFR Part 11 compliance.
Bruker Daltonics also offers optional IQ, OQ and PV services to FDA-regulated customers.
