Designed for purification or removal of highly hydrophobic proteins applied in the industrial-scale purification of recombinant hepatitis B surface antigen vaccine
GE Healthcare has announced the availability of the Butyl-S Sepharose 6 Fast Flow, a hydrophobic interaction chromatography (HIC) medium designed for purification or removal of highly hydrophobic proteins applied in the industrial-scale purification of recombinant hepatitis B surface antigen vaccine.
"The control of hepatitis B is one of the biggest public health challenges in the world today with the World Health Organisation estimating that the total number of chronic carriers of hepatitis B could be as high as 350 million with about one million deaths per year associated with the virus", commented Lars-Johan Larsson, director product management media, protein separations, at GE Healthcare.
"Butyl-S Sepharose 6 Fast Flow provides a key step in the downstream purification process for hepatitis B vaccine and offers on-line analysis during purification"" The capture step utilising adsorption of hepatitis B surface antigen integrates easily with subsequent chromatographic steps for intermediate purification and polishing, and with ultrafiltration (membrane filtration) steps for buffer exchange and product concentration.
In addition to being utilized in the production of the hepatitis B vaccine, Butyl-S Sepharose 6 Fast Flow can be used in the development or manufacture of other recombinant viral vaccines or virus-like particles or (VLPs), as well as in the removal of strongly hydrophobic biomolecules from feedstocks.
