With electron transfer dissociation in a high-capacity ion trap, protein scientists can sequence peptides and simultaneously identify both the type and location of post-translational modifications
Bruker Daltonics announces a commercial electron transfer dissociation (ETD) module for its top-of-the-line high-capacity ion trap mass spectrometer, the HCTultra.
ETD is described as a unique new peptide and protein fragmentation technique that preserves functionally important post-translational modifications (PTMs), such as phosphorylation or glycosylation.
With ETD in a high-capacity ion trap, protein scientists can sequence peptides easily, and in addition they can simultaneously identify both the type and location of various PTMs.
Detlev Suckau, head of proteomics applications development at Bruker Daltonics, commented: "Modern proteomics has moved beyond simple identification, and one of the primary goals of functional proteomics is now the unravelling of the many subtle modifications arising in proteins which often are very important for their biological activity".
""By making ETD capabilities available broadly to all protein scientists for specific PTM analyses on the ultra-high performance HCTultra ion trap, we continue our leadership in the area of top-down proteomics, where we have previously pioneered important related developments such as highest-performance commercial ECD on our FTMS, and our unique T3-sequencing on the Ultraflex Tof/Tof"".
"ETD MS/MS spectra of peptides can now be collected on-the-fly during LC/MS/MS runs using the HCTultra".
"Due to its non-ergodic nature, ETD typically creates very clean MS/MS spectra with intact PTMs, says Bruker".
"On the HCTultra, the new ETD implementation often provides complete amino acid series down to immonium ion masses, without the low-mass cut-off traditionally encountered in ion trap MS/MS.
The combination with the superior mass accuracy of the HCTultra enables powerful de novo sequencing capabilities".
"Using the PhosphoScan mode of the HCTultra with ETD offers a unique and powerful system for single run auto-LC/MS/MS/MS CID/ETD experiments specifically for phosphorylation analysis".
"Michael Schubert, vice president of R and D, stated: "The market-leading acquisition speed and sensitivity of the HCTultra combined with the new information-rich ETD MS/MS capability redefine the ion trap standard for the detection and localisation of post-translational modifications, as well as for de novo sequencing of peptides".
""Its high ion capacity and dynamic range make this system also especially suitable for quantitative proteomics".
ETD and CID data are supported by Bruker Daltonics and bioinformatics suite ProteinScape, which was selected by the Hupo Brain Proteome project as an official tool.
It can conveniently handle large numbers of high-quality MS/MS spectra created by the HCTultra in proteomics studies.
The HCTultra seamlessly integrates into the Proteineer solution for LC/MS/MS and 2D gel-MS/MS based proteomics projects, as well as into the Metabolic Profiler solution for metabolic studies.
