Protein fusions can be arranged to add a domain, protein, or localisation signal, in order to overcome the mismatch between commercially available fluorescent reporters and experimental conditions
The mismatch between commercially available fluorescent reporters and the particular customer's experimental conditions lowers down the efficiency of these reporters' usage, says Evrogen.
To overcome this, the company has introduced a service supplying vectors encoding Evrogen fluorescent reporters fully optimised according to customer requirements.
Protein fusions can be arranged to add a domain, protein, or localisation signal of interest to the Evrogen fluorescent tag.
Cloning of required promoters into promoterless vectors encoding Evrogen fluorescent proteins can also be arranged allowing the analysis of the promoter activity.
Codon usage of the desired fluorescent protein can be optimised for best expression in heterological system of customer's interest.
As well as this, fluorescent proteins can be cloned into specific vectors matching customer's needs.
The combination of the two previously-separate services - the production of fluorescent reporters, and their conditional optimisation - allows Evrogen customers to save time, money and efforts on adjusting the reporter to function optimally, the company says.
A well-developed system of discounts is also provided.