Protein refolding kits use reagents which ensure these proteins can refold to their original form and so significantly improve yield by preventing aggregation
Novexin introduces Refold Master, its range of protein refolding kits.
These generic laboratory scale kits work with all proteins using just one set of conditions, says the company.
This saves time and effort with screening and allows scientists that normally avoid working with insoluble proteins to progress their research with ease.
Refold Master uses Novexin's proven technology and non-detergent based reagents, which work to protect insoluble proteins during vulnerable stages in the refolding process.
The reagents ensure these proteins can refold to their original form and so significantly improve yield by preventing aggregation while allowing increased concentrations of proteins to be refolded.
Additionally, since proteins generated by the kit refold properly, they are active and have the correct 3D structure, which means users benefit by saving time screening multiple refolding conditions and by eliminating post-purification procedures.
The inexpensive Refold Master kits comes complete with easy to follow instructions and, uniquely according to Novexin, have everything scientists will need for refolding and protein purification, including media, columns and membranes for polishing steps.
The kit is available in three sizes, which allow refolding of 0.5mg, 5mg and 50mg of the target protein.
Users can also choose kits for acidic, basic or his-tagged fusion proteins with the 5mg and 50mg Refold Master kits.
The reagents within the kit are scalable so that when a laboratory-based protocol is established, this can easily be transferred to process scale-up to increase yields and reduce manufacturing costs.
Daniel Jones, CSO of Novexin commented: "We are delighted to launch an affordable laboratory kit because it allows all scientists involved in protein processing access to our unique technology.
"Since Refold Master kits use reagents that will improve yields of most proteins, scientists can significantly reduce the time they spend screening refolding conditions, as well as cut down their protein purification runs, thus saving them hours of valuable research time."
