Advanced analysis software module for ratiometric assays enables quick determination of percent conversion and percent inhibition for compounds in high-throughput screening (HTS)
Nanostream, a provider of high-throughput microfluidic analytical systems to companies involved in drug discovery and development, announces the availability of an advanced analysis software module for ratiometric assays.
Said to be ideal for scientists in high-throughput screening (HTS), the ratiometric assay module helps researchers process large amounts of data and rapidly determine percent conversion and/or percent inhibition for compounds using separation-based assay data from Nanostream's Veloce micro parallel liquid chromatography (uPLC) system.
Nanostream says it created each advanced analysis software module with the intent to streamline routine data manipulation and speed time-to-results.
The ratiometric assay module is designed to calculate the percent conversion of any sample with two peaks, eg, a product peak and a substrate peak, in a separation-based assay.
These analyte peaks have known retention times, and the peak area ratios are tabulated using user-defined time windows.
When researchers need to run samples along with fully reacted controls, the percent conversion values are calculated and an average is computed for all control samples.
After rapidly computing each peak area ratio, a summary displays the percent conversion and inhibition for each sample in addition to each sample's product and substrate retention times and peak areas.
"Assay variability is a major concern for HTS scientists.
"They work day and night to minimise variability and improve lead-to-hit ratios, which can reduce the expense associated with the pursuit of false positives", said Hau H Duong, vice president of engineering, Nanostream.
"The advanced analysis software module for ratiometric assays is an integral part of Nanostream's solution to help researchers reduce interferences and improve data quality early in drug discovery".
Last March, Nanostream introduced its suite of advanced analysis software modules to support a range of analytical applications, including compound library purity, logP, logD, solubility and permeability.
Nanostream's analysis software comes standard with the Veloce system, and the advanced analysis modules are available for an additional cost.
Nanostream provides high-throughput microfluidic analytical systems to companies involved in drug discovery and development.
Nanostream's systems are based on proprietary, modular product design that allows for timely introduction of new products based on market needs.
Nanostream is pioneering micro parallel liquid chromatography (uPLC) with its premier product, the Veloce system.
The Veloce system, designed for scientists who can benefit from greater sample analysis capacity, integrates with existing workflow and increases the throughput of established separation techniques.
