Tangential flow filtration (TFF) products allow researchers to concentrate and desalt proteins from cell culture supernatants and fermentation broths
Millipore has announced two tangential flow filtration (TFF) products that allow researchers to easily and efficiently purify large volumes of protein-containing solutions.
Applications typically include concentrating and desalting proteins from cell culture supernatants and fermentation broths.
The high flux, low binding Millipore membranes used in these devices provide high protein retention and recovery.
Pellicon XL devices are available with a choice of either Durapore PVDF membrane (microporous), Ultracel regenerated cellulose membrane (ultrafiltration) or Biomax polyehtersulphone membrane (UF), and membranes for concentrating and desalting from 100 millilitres to two litres of protein-containing solution.
Compared to large stirred cell systems, Pellicon XL devices offer better flow rates with less processing time, says Millipore.
Additionally, the devices can be docked with the Labscale TFF pump system without the need for tubing or clamps.
Pellicon 2 mini-cassettes are available with either regenerated cellulose, or polyethersulphone membranes for processing up to ten litres of protein-containing solution.
Larger cassette systems are available for processing 250 litres or more of solution.
Tangential flow filtration systems are designed specifically for filtering larger volumes of solution.
In traditional filtration systems, fluid is directed toward the membrane with applied pressure.
When processing larger volumes, retained molecules can build-up on the surface of the membrane and slow, or block, filtration.
In TFF, fluid is pumped tangentially along the surface of the membrane.
An applied pressure forces smaller molecules through the membrane, while larger molecules are swept along the surface of the membrane, which prevents the membrane from fouling.
