Single-use spin column for purification of histidine-tagged proteins allows direct loading of unclarified homogenised cell lysates, without the need for centrifugation or filtration of the sample
GE Healthcare has announced the availability of the His SpinTrap, a single-use spin column designed for efficient purification of histidine-tagged proteins using a standard microcentrifuge.
His SpinTrap allows direct loading of unclarified homogenised cell lysates, without the need for centrifugation or filtration of the sample.
His SpinTrap saves researchers valuable time as it eliminates the need for tedious sample preparation and enables direct protein purification in as little as ten minutes per run.
By reducing the total purification time, the spin column procedure minimises degradation and oxidation of sensitive protein samples and preserves the activity of the target protein.
"GE Healthcare's NI Sepharose products have been warmly received by researchers in life sciences who purify histidine-tagged proteins," said Allan Simpson, vice president of research and analysis, protein separations at GE Healthcare.
The products are available in prepacked columns for use with chromatography systems and as bulk packs.
GE has recently expanded the product range to enable researchers who prefer working with spin columns, multi-well plates, and gravity columns to take advantage of the great benefits of Ni Sepharose such as outstanding binding capacity, negligible nickel ion leakage, and tolerance to additives".
His SpinTrap is packed with Ni Sepharose High Performance, which provides a high binding capacity-up to 750ug of pure histidine-tagged protein per column-and compatibility with a variety of commonly used additives and denaturing and reducing agents.
In addition to its use for small-scale protein purification, the columns are also ideal for screening large numbers of small-scale bacterial lysates and for optimizing protein purification conditions.
