In an article in the latest issue of Biotech International cell densities are evaluated using imaging of crystal violet-stained cell layers, phase contrast, and fluorescence microscopy
Ibidi has published new data on homogeneous cell growth.
The data are based on Ibidi's microscopy carriers, the u-Slides, and show superior cell distribution and phase contrast images over the entire cell adhesion area - a significant advantage compared to open well culture systems.
The u-Slides are carriers for the cultivation and subsequent imaging of cells.
In an article in the latest issue of Biotech International cell densities are evaluated using imaging of crystal violet-stained cell layers, phase contrast, and fluorescence microscopy.
Cell cultures in microchannels have been compared with open well cultures, using similar adhesion areas.
In open wells, the meniscus formed at the walls seriously affects the homogeneity of the cells.
In contrast, cells inside channels of u-Slides always appear perfectly homogeneous distributed.
Ibidi's responsible project manager, Elias Horn, commented: "The homogeneity inside the channels is a consequence of the microfluidic properties of the u-Slides, in particular the height of the channel being only 0.4mm.
"Therefore, highly defined cell densities can be utilised in order to measure cell proliferation, and cell cycle times.
"Consequently, the high optical quality of the u-Slides allows their utilisation for cell based assays in research and high throughput applications".
Ibidi develops, produces and distributes micro-slides (u-Slides), a 'lab-on-a-slide' technology for the analysis of cells and biomolecules.
The u-Slides represent a new family of flow through devices for the functional analysis of living cells.
Currently, the u-Slides product family comprises eight different slide types for cell-culture and optical microscopy (high resolution, fluorescence, DIC) and protein-binding.
