The Chromatrap Enzymatic Shearing Kit is designed for easy preparation of high quality, ideally fragmented chromatin for ChIP analysis.
The success of a ChIP assay is highly dependent on the quality of chromatin prepared. The Enzymatic shearing kit provides buffers to optimise the shearing conditions of chromatin by enzymatic digestion.
Chromatin prepared using the kit is claimed to demonstrate excellent enrichment, independent of starting cell number. It is designed to be a quick, simple and cost-effective alternative to sonication.
The Kit contains reagents and buffers for up to ten chromatin preparations. This generates enough chromatin to perform up to 24 ChIPs using a standard Chromatrap ChIP spin column kit or up to 96 IPs using the Chromatrap 96 HT microplate kit.
