Chromatrap has introduced an improved version of its solid phase chromatin immunoprecipitation (ChIP) assay kit for chromatin extracted from Formalin Fixed Paraffin Embedded (FFPE) tissue.
Suitable for tissue samples, biopsies and difficult to lyse samples, kit features a streamlined protocol which provides the user with excellent quality isolated chromatin. Its protocol is shorter, offers increased chromatin yield/accessible epitopes and provides greater flexibility in chromatin sample loading for ChIP.
The kit is compatible with both qPCR and sequencing as a downstream process and has a wide dynamic range, ensuring that adequate DNA is enriched for library preparation for sequencing. Its wide dynamic range enables better results from small sample sizes and provides greater flexibility. DNA purification prior to sequencing can be conducted using the supplied Chromatrap purification columns.
Each Chromatrap FFPE ChIP-seq kit contains sufficient Protein A or Protein G based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitation assays and up to 10 chromatin preparations.
Product Features:
- Sensitivity of the unique solid state platform ensures that small samples can be used with efficient enrichment of high and low abundant targets.
- Quick, easy and efficient way of performing ChIP assays from a range of FFPE samples.
- Maximises the capture efficiency of the target chromatin/antibody complex. By avoiding the use of magnetic or agarose beads, non-specific background is eliminated together with reduced manual handling errors and subsequent sample loss.
