Porvair Sciences’ P3 microplate can be used to accelerate removal of interfering proteins from serum, plasma or even whole blood samples prior to analysis by Liquid Chromatography/Mass Spectrometry (LC/MS).
Biological samples commonly contain proteins that interfere with downstream applications. Traditionally, scientists have used the CRASH method, in which the protein is denatured with acetonitrile and the flocculant filtered out to clean their samples prior to LC/MS analysis.
With the P3, protein ‘crashes’ out of solution and precipitates directly in each well of the microplate when acetonitrile is added, thus solving problems traditionally associated with the CRASH technique of protein clean-up. The novel dual frit, hydrophobically treated matrix used by the P3 microplate means that there is no ‘wetting out’ or leakage of sample before the application of vacuum. Using the plate enables 96 samples to be filtered simultaneously – accelerating LC/MS protocols.
