Porvair Sciences has launched version 2.0 of its Chromatrap ChIP-seq kit - developed to perform chromatin immunoprecipitation (ChIP) with unparalleled sensitivity.
The kit enables researchers to enrich chromatin, detecting binding of low abundant transcription factors and histone modifications from a small quantity of starting material (1000 cells or 50ng).
Using proprietary bead-free technology in parallel with high throughput sequencing, the kit requires fewer manual handling steps than its predecessors, making it suitable for first time ChIP users.
Up to 24 ChIP assays can be performed, from cell collection through to immunoprecipitation, including up to ten chromatin sample preparations. All of the major components required for performing ChIP assays to obtain high quality DNA for Next Generation Sequencing library preparation or qPCR experiments are included.
Notable developments for Version 2 include the ability to achieve high quality chromatin via sonication or enzymatic digestion, improved antibody binding, greater chromatin loading flexibility and the ability to use increased slurry volumes for difficult samples. A complete ChIP-to-Sequencing protocol can be completed in five hours.
