ChIP Kits for High Throughput Antibody Validation

Chromatrap’s bead-free systems aims to offer a fast, easy and sensitive method of validating antibodies in a high throughput format. This, in turn enables antibody suppliers to easily and efficiently expand their product portfolio to target epigenetics and drug development applications.

Successful ChIP assays rely heavily on the highest quality antibodies. In-house validation of antibodies for use in ChIP is often considered to be a lengthy, expensive, time consuming and challenging process. In addition, antibodies are rigorously assessed for enrichment of positive and negative gene targets, signal-to-noise ratio, chromatin-to-antibody ratio, reproducibility and lot-to-lot variations.

Dr Lindsay Parkes, a senior research scientist at Porvair Sciences said: “We have developed our bead-free Chromatrap technology to enable maximum capture of antibody. This is achieved by correctly orientated capture proteins throughout the inert filter-based ChIP technology. Most bead-based systems have capture proteins scattered in random orientation which doesn't allow full access of antibodies to binding pockets of proteins, thus giving an overall reduced capture efficiency. This is important for high sensitivity especially for detection of binding of low abundant targets.”

Chromatrap ChIP kits deliver fast workflow, enabling users to process up to 96 ChIP assays simultaneously in one day – allowing multiple sample, antibody and gene targets to be tested in parallel on a single plate. Requiring minimal manual handling and pipetting steps, the kits are designed for easy use, making them suitable for even inexperienced users. Using these kits, which are suitable for use over a wide chromatin enrichment range (500ng - 50µg), a complete ChIP protocol can be completed in less than five hours.

Find further information CLICK HERE or contact Porvair Sciences Ltd on +44-1978-661144 / support@chromatrap.com