Effective Buccal Swab RNA Extraction for COVID-19 Diagnostic Testing
25 Aug 2020
Porvair Sciences announces a special version of its Homogeniser Spin Column, optimised for RNA extraction from buccal swabs.
Many COVID-19 diagnostic tests are reliant on detection of viral RNA from buccal swabs by PCR. The Chromatrap Homogeniser Spin Column from has been adapted to ensure that it can routinely improve yield and quality of viral RNA from nasal and throat swab samples.
Whether researching COVID-19 to understand its mechanisms of action, or as part of a frontline diagnostic testing facility, the instrument provides a fast procedure for downstream RNA extraction, achieving great results in just minutes. It’s dual frit extraction design reduces lysate viscosity and captures insoluble debris by centrifugation. The homogenised lysate sample is then ready for RNA extraction.
Using the Column increases RNA yield compared to alternative shredding columns or if no homogenisation is undertaken. It also provides faster homogenisation than traditional methods using syringe and needles, with reduced waste and minimal loss of sample. Every COVID-19 RNA extraction has its own column, eliminating sample cross contamination and the possibility of false positive diagnosis.
The Chromatrap Homogeniser Spin Column is compatible with all manual RNA extraction procedures including the Qiagen RNeasy and Invitrogen RNA link extraction kits.