High performance microplates for enhanced cell growth and survival
25 Apr 2022
The Sero Krystal PDL range of coated microplates from Porvair Sciences are designed to help overcome the challenges of reproducible cell culture, even with challenging cell types.
Whilst many scientists would prefer to grow cells in a serum-free environment to more accurately mimic physiological conditions, cell lines can struggle to attach to tissue culture plastics in the absence of serum.Manufactured from ultra-pure polystyrene in a clean room environment, SeroKrystal Poly-D-Lysine (PDL) coated microplates were developed to promote superior cell attachment, growth and proliferation for a wide range of cell types in serum-free media.
Poly-D-Lysine is a synthetic (animal-free) extracellular matrix used in cell culture applications to promote cell adhesion. Available in 96- and 384-well formats the microplates are uniformly coated with a 70 to 150 kDa PDL polymer using proprietary methods. The coating creates a uniform net positive charge on the surface of the plate wells which improves electrostatic interactions between the coated surface and the negative charges of the cell membrane and improves cell attachment.
The plates are suitable for the culture of transfected cell lines, primary neurons, glial cells and fibroblasts, such as the adherent mouse fibroblast cell line L929 and other common adherent cell lines including HEK-293, BHK-21, PC12 and, NSC-34. They are available in clear, black and frame versions and incorporate an optically clearbase.
The consistent flatness of the Sero Krystal microplate base is designed to provide superior light transmission for high resolution imaging and a flat optical plane for growing cells. Supplied sterile and ready-to-use, they are the first part of the new Sero range of cell culture plates to launch from Porvair Sciences.