Combination of a fibre-optic coupled micro cell with a range of high performance UV/Vis spectrophotometers provides highly accurate reproducible analysis of nanodroplets as small as 70nl
Lambda Advanced Technology offers a new system for spectrophotometric analysis of 1ul biological samples.
The combination of a fibre-optic coupled micro cell with a range of high performance UV/Vis spectrophotometers provides highly accurate analysis of nanodroplets as small as 70nl with remarkable reproducibility.
The average dynamic range for dsDNA is between 2ng/ul and 5000ng/ul, depending on the particular spectrophotometer.
A choice of spectrophotometers from low cost reference beam systems to double beam research quality instruments brings unparalleled versatility.
Although all the instruments feature comprehensive nucleic acid application programs, this powerful combination is not restricted to nucleic acid measurements.
They retain their full spectrophotometer capabilities and can be used both with standard and microlitre samples.
Standard nucleic acid measurements typically include absorbance ratio (A260/280), purity and concentration measurements for RNA, ssDNA and dsDNA.
Oligonucleotide molar concentrations can also be calculated.
Protein concentration is calculated using the Warburg-Christian method.
Labelled DNA measurements can also be made.
By calculating the concentration of unlabelled primer (oligomar) and primer labelled with a fluorescent dye, the combination can be used to verify that the dye is bound to the primer in advance of DNA sequencing applications.
Two versions of the micro cell are available giving a defined optical light path of 1mm and 0.2mm.
Samples are dropped onto the surface of the cell's optical window.
Integrated beam deflection and fibre- optic cables allow direct measurement.
A sample cap equipped with a mirror completes the optical arrangement and prevents the sample from drying up.
In addition, valuable samples can be retrieved after analysis.
